MiR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Huichao Wu¹, Lin Zha², Huanyu Guo³, Yingjie Xie⁴, Jianhua Hu⁵, Xinxia Tan⁶
¹Department of Emergency, The First People’s Hospital of Jiashan, Jiashan 314100, China
²Department of Hematology and Oncology, Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing 100010, China
³School of Clinical Medicine, Changchun University of Chinese Medicine, Changchun, 130117, China
⁴One Departments of Cadre Ward, General Hospital of Southern Theater Command of Chinese PLA, Guangzhou, 510010, China
⁵Department of Intensive Care Medicine, People’s Hospital of Quzhou, Quzhou, 324000, China
⁶Department of Clinical Laboratory, Huangshi Central Hospital (Affiliated Hospital of Hubei Polytechnic University), Huangshi 435000, China; Huangshi Tumor Molecular Diagnosis and Treatment Key Laboratory, Huangshi 435000, China

Objective: Acute myeloid leukemia (AML) is a frequent and highly lethal hematological malignancy that is difficult to treat. The research aimed to clarify the molecular mechanisms of MIR-379-5p in AML progression.
Materials and Methods: RT-qPCR was utilized to evaluate MIR-379-5p expression levels in AML patients and a control group. A ROC curve was created to assess the clinical predictive value of MIR- 379-5p in AML, while cell experiments used CCK-8 assay, flow cytometry, and Transwell chambers. Predicted potential target genes of MIR-379-5p by employing online bioinformatics tools, followed by validation using a dual luciferase reporter assay
Results: MIR-379-5p was significantly decreased in AML patients and had clinical predictive value for the disease. In AML cell lines, MIR-379-5p was down-regulated; conversely, the up-regulation of MIR- 379-5p inhibited proliferation, migration, and invasion while promoting apoptosis. Notably, YBX1 was a potential target gene of MIR-379-5p, and its upregulation reduced the effects of MIR-379-5p on AML cell behavior.
Conclusion: MIR-379-5p had the potential as a biomarker for AML by regulating cell proliferation and apoptosis through targeting YBX1.

Keywords: AML, MIR-379-5p; YBX1; cell proliferation; apoptosis; diagnostic significance

Sorumlu Yazar: Huichao Wu

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