The effects of chlorhexidine-based preservative tubes on routine urinalysis stabilityInanc Karakoyun1, Fatma Demet Arslan1, Anil Baysoy1, Murat Aksit2, Ayfer Colak1, Banu Isbilen Basok11Department of Medical Biochemistry, University of Health Sciences, Tepecik Training and Research Hospital, Izmir, Turkey 2Department of Medical Biochemistry, Giresun University, Prof. Dr. A. Ilhan Ozdemir Training and Research Hospital, Giresun, Turkey
INTRODUCTION: To evaluate the effects of chlorhexidine-based preservative tubes on analytes for routine urinalysis. METHODS: Urine specimen (n=84) aliquots in polystyrene tubes (PS) with no additives (Fıratmed Co. Ltd., Istanbul, Turkey) and in Becton–Dickinson Vacutainer® Plus Urinalysis Preservative (BD-UAP) tubes (Becton Dickinson Inc., NJ, USA) were analyzed on automatic modular urine analyzer (Dirui Industry, Changchun, China) and the results were compared. Stability was assessed by re-analyzing urine samples in BD-UAP tubes after three and six hours of storage and comparing with initial results. Cohen’s kappa coefficient (κ) was calculated to assess the agreement of results. RESULTS: Squamous epithelial cell and pH agreement between the tubes was moderate (κ=0.60 and 0.58, respectively). Bilirubin, ketone, protein, nitrite, and glucose showed perfect agreement with the PS tube and remained stable for up to six hours in the BD-UAP tube (κ=1.00, 0.92, 0.83, 0.93, 1.00, respectively). Red blood cells, white blood cells, bacteria, blood, and leukocyte esterase showed moderate to substantial stability at six hours (κ=0.56, 0.77, 0.76, 0.87, 0.57, respectively). DISCUSSION AND CONCLUSION: Although storage in chlorhexidine-based preservative tubes has limited effects on chemical strip analysis, these tubes may not be considered optimal for microscopic examination.
Keywords: Chlorhexidine, preanalytical phase, storage, urinalysis, urine
Inanc Karakoyun, Fatma Demet Arslan, Anil Baysoy, Murat Aksit, Ayfer Colak, Banu Isbilen Basok. The effects of chlorhexidine-based preservative tubes on routine urinalysis stability. Int J Med Biochem . 2020; 3(3): 155-160
Sorumlu Yazar: Inanc Karakoyun, Türkiye |
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