The Effect of Infliximab on Oxidative Stress in Ovarian Tissue in the Rat of Ovarian Hypersrimulation Syndrome

INTRODUCTION: Ovarian hyperstimulation syndrome (OHSS) is a complication that occurs during assisted reproductive techniques. In this study, our aim is to study the effect of Infliximab (IFX) on oxidative stress in ovarian tissue in a rat model of ovarian hyperstimulation syndrome.
METHODS: A total of 32 immature female rats were divided into four groups randomly: Control, OHSS, OHSS+IFX, and IFX group. OHSS and OHSS+IFX groups were administered 30 IU pregnant mare serum gonadotropin/day on days 22-25 of life and 30 IU hCG on day 26. Plus, intraperitoneal IFX was administered half an hour before hCG administration to the OHSS+IFX group. On the 26th day, solely 7 mg/kg IFX was administered to the IFX group. 48 hours after administration of hCG administration, the rats were sacrificed and their ovarian tissues were sampled. The levels of MDA, AOPP, TSG as well as catalase activity were measured in these ovarian tissues.
RESULTS: Ovarian tissue MDA and AOPP levels of the OHSS group were determined to be significantly higher compared to the control and OHSS-IFX groups. Ovarian tissue catalase activity and TSG level of the control group was significantly higher compared to the OHSS and OHSS-IFX groups. It was determined that although ovarian tissue catalase activity and TSG level in the OHSS-IFX group were higher compared to the OHSS group but it was not significant.
DISCUSSION AND CONCLUSION: The results revealed that IFX could prevent oxidative stress in ovarian tissue induced by OHSS. These effects may be mediated by the anti-inflammatory and antioxidant properties of IFX.