Investigation of antibiotic resistance genes of beta-hemolytic and non-hemolytic streptococci with molecular methods

INTRODUCTION: Infectious diseases have been one of the most important problems affecting all societies since the existence of mankind. Streptococci are an important cause of diseases in humans such as meningitis, acute otitis media, rhinosinusitis and community-acquired pneumonia.
METHODS: In this study, various clinical samples belonging to the disease were taken between January and September 2015. Analyzes of determined and identified antibiotic resistance genes of isolated and identified streptococci strains were carried out in the Microbiology laboratory of Yüzüncü Yıl University Faculty of Pharmacy by PCR. The detection of pbp1a for penicillin G antibiotic, pbp2x for cefotaxime antibiotic, and gyrA resistance genes for quinolone antibiotics were targeted. In this study, the primers used in Table-2 were taken as reference.
RESULTS: A total of 30 streptococcus strains were isolated and identified from various clinical samples including 8 sputum, 6 urine, 5 BOS, 4 blood, 4 nasopharyngeal swab, 2 ear effusion and 1 abse. As a result of the PCR analysis, the resistance of pbp1a was found in 6 of the streptococcal isolates, pbp2x in 8, and resistance in the gyrA gene region in 5. In our study, no three resistance genes were found in 22 isolates and the presence of all three (pbp1a, pbp2x and gyrA) resistance genes in 5 isolates was determined.
DISCUSSION AND CONCLUSION: It is expected that the data obtained from this research will contribute to national and international knowledge accumulation.