[doi: 10.5505/2017ichc.PP-28]Comparison of the healing effects of 3 different antioxidants on Cisplatin-induced pulmonary toxicity: A immunohistochemical and stereological studyTugba Bal1, Nurhan Akaras1, Serdar Yigit2, Hilal Atilay1, Ozlem Ozgul Abuc1, Elif Polat12Department of Histology and Embryology, Kafkas University, Kars, Turkey Introduction & OBJECTIVES: Chemotherapy, which has a great importane to cancer, is a treatment method. Many agents have been developed for use in chemotherapy treatment. Cisplatin (cis-diamminedichloroplatinumⅡ, CDDP), one of these agents, has many side effects despite of therapeutic. Previous studies reported that cisplatin would toxic effects on many systems including pulmonary system. Many active ingredients are used to reduce the effect of toxic substances and to protect cells. In the present study we focused on Curcumin (CMN), Cape ( Caffeic acid phenethyl ester) and Amifostine (ethiole) antioxidants. Aim of the this study was to compare the protective effects of Curcumin, Cape and Amifostine on Cisplatin-induced pulmonary toxicity via histopathological, immunohistochemical and stereological methods. Materials & METHODS: 200 -300 g weighing, 30 Sprague-Dawley male rats were used in the experiment. The rats were randomly divided into five groups of six numbers as follows: (1) Control group, (2) Cisplatin group (5mg/kg-ip), (3) Cisplatin + CAPE (10 μmol/kg-ip), (4) Cisplatin + Amifostine (400 mg/kg-ip) and (5) Cisplatin + Curcumin (10 mg/kg-gavage). At the end of the experiment lung tissue samples were taken from all rats. Sections of 4 to 5μm thickness taken from each block were stained with Hematoxylin-eosin (H-E) and Caspase-3 Ab. All sections were stereologically evaluated using random sampling and fractionator method and immunopositive alveoler wall cell density was calculated. Statistical significance of the results (SPSS-19, one-wayANOVA) was evaluated according to p <0.05. RESULTS: In the cisplatin-treated group, capillary dilation, septal thickness, inflammatory cell infiltration and erythrocytes extravagation were observed when compared to the other groups. After cisplatin injection, it was significantly detected to inhibit the side effects caused by cisplatin in the Curcumin, Cape and Amifostine treated groups. These findings were supported immunohistochemical and stereological analysis as well. Although immunopositivity was significantly lower in Cisplatin + Amifostine and Cape groups than in the cisplatin group, no significant difference was found in the Curcumin group. Additionally, positive cell density in the Amifostine group was lower than in the Cape group. CONCLUSIONS: Our study revealed clearly that cisplatin-induced pulmonary damage was significantly reduced by Amifostine and Cape antioxidants. |