[doi: 10.5505/2017ichc.PP-258]

The Effect of Thermal Energy on The Neovascularızatıon After The Saphenofemoral Compound Lıgatıon and Saphenous Veın Strıppıng

¹Vocational School of Health Services, Mersin University, Mersin, Turkey
²Department of Histology and Embryology, Faculty of Medicine, Inönü University, Malatya, Turkey
³Department of Cardiovascular Surgery, Faculty of Medicine, Mersin University, Mersin,Turkey

Introduction & OBJECTIVES: It is known that the two most important factors in the recurrence of varicose veins after varicose vein surgery are stump and neovascularization in the saphenous femoral junction (SFJ). The aim of this experimental study is to contribute to the elucidation of the etiopathogenesis of recurrence and to investigate the effect of thermal energy on neovascularization in SFJ divisions in rats.
Materials & METHODS: Seven adult wistar rats have been used for each group in the study. The rats were named Group 1(surgical) and Group 2 (thermal energy- laser), respectively, after right femoral venous binding with prolene, then with scissor divide and directly with cotter divide. The left legs of the same rats without any attemp were accepted as the control group. After routine light microscopic tissue processing; rat tissue samples were stained by Hemotoxylin-Eosin and VEGF and also with S100 for neovascularization determination. In light microscopic sections, capillaries were counted with 10 different field x20 lens magnification and the average was taken and H-Scores were determined. For the statistics analysis was applied the Spousal T Test and Kolmogorov-Simirnov Test.
RESULTS: In light microscopic examinations, capillary counts were made for neovascularization and H-Ssors were detected. As a result of statistical analysis; It was observed that each group applied the Kolmogorov-Simirnov test was normally distributed separately (p> 0,05). In VEGF immunostaining; while there was statistically significant difference between Control and Group 1 (p: 0,001), between Group 1 and Group 2 (p: 0,039), there was no statistically significant difference between Control and Group 2 (P: 0,199). When S100 staining was compared, there was a statistically significant difference in H-score between Control-Group1 (p: 0.001), between Group1 and Group2 (p: 0.003) and between Control-Group2 (P: 0.006).
CONCLUSIONS: In our study, it was observed that the use of thermal ablation in divisions of varicose vein surgeon in SFJ reduced neovascularization emerging in varicose vein recurrence. In our study, results that supporting endovenous thermal treatments have been reached. Thermal energy applications may be an easily applicable, minimally invasive and alternative way of preventing varicose venous recurrences.

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Table 1

	Control (n=7)	Group1 (Surgical group, n=7)	Group2(laser group n=7)
	mean±SD	mean±SD	mean±SD
VEGF	31,428 ± 8,997	88,571 ± 24,784	47,142 ± 24,299
S100	41,428 ± 14,638	92,857 ± 21,380	71,428 ± 22,677

Table 1. Comparison of baseline characteristics between the controls and other groups