[doi: 10.5505/2017ichc.PP-239]

Protective effect of Caffeic acid phenethyl ester against cisplatin-induced nephrotoxicity in rats

Tayfun Ceylan, Emin Kaymak, Betül Yalçın, Birkan Yakan
Depatment of Hsitology and Embriyology, Erciyes University, Kayseri, Turkey

Cisplatin (CP), an antineoplastic drug made in the end of the 19th century (1). Cisplatin may cause ototoxicity, nephrotoxicity, myelosuppression, and peripheral neuropathy (2). Also cisplatin leads to Inflammation, oxidative stress, and apoptosis in nephrotoxicity (3,4).
Caffeic acid phenethyl ester (CAPE) is a component of propolis, a honey bee product (5). CAPE is used in traditional medicine. CAPE has protective properties for many tissues, has antiviral, anti-inflammatory, immunomodulatory, and antioxidant proporties. CAPE inhibits lipid peroxidation, lipoxygenase and cyclooxygenase enzymes (6-10).
The aim of this study is to evaluate the effects of caffeic acid phenethyl ester on nephrotoxicity induced by cisplatin.
A total of 40 male rats were equally divided into four study groups namely, control, CP (7mg/kg for 7 th day), CAPE (10 µmol/kg /day for 10 days) and CP+CAPE (7mg/kg for 7 th +10 µmol/kg /day for 10 days). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue superoxide-dismutase (SOD), catalase (CAT) and the level of malondialdehyde (MDA) and histopathological damage scores were then compared.
İn CAPE group, tissue CAT and SOD levels were higher than control group. When SOD and CAT levels were measured, no significant difference was observed between CAPE and CP+CAPE groups. İn CP group, tissue MDA levels were higher than control group, but interestingly, the dose of CAPE we administered increased the level of MDA in the kidney. İn CP group, tubules observed dilated with thin denuded lining epithelium, atrophy when compared with control group. CAPE treated group showed showed an decreased histological appearance in the CP group.
Our results suggested that CAPE increases antioxidant activity, while it causes lipid damage as dose-dependent in kidney tissues. CAPE can be protective effect against tubular damage.