[doi: 10.5505/2017ichc.PP-238]
The Effect Of Systemic And Local Minocycline On The Fat Graft Survival And The Inhibition Of Apoptotic Pathway
Kırdar Güney1, Serhan Tuncer1, Bora Özel1, Çiğdem Elmas2, Cemile Merve Seymen2, Seyhan Çenetoğlu12Department of Histology and Embryology, Medical School, Gazi University, Ankara, Turkey
INTRODUCTION: Recently use of fat grafting has been popularized in plastic surgery. Because it is good soft tissue filler, cheap and easily obtained and has no side effects so these are the main reasons for popularized using. But the survival rate of fat grafts is unknown.
AIM: The purpose of this study to increase fat graft survival rate and to inhibit early stage apoptosis that cause fat cell death after ischemia by using minocycline.
METHODS: 48 Wistar Albino rats were divided into 8 groups. Group 1 and 5 are control groups. Group 2 and 6 are systemic minocycline groups. Group 3 and 7 are local minocycline groups. Group 4 and 8 are local and systemic minocycline groups. In every group, inguinal fat pads were got and after systemic, local or both minocycline application, fat pads transplanted animal’s back. Group 1, 2, 3 and 4 were sacrifice after 90 days later. Group 5, 6, 7 and 8 were sacrifice after 9 days later and all fat pads determined by Hematoxylin Eosine, 90. days groups stained by Oil Red O and 9. days groups examined by TUNEL stainings for all groups. Results also analysed using statistical methods.
RESULTS: Grafts treated with local and systemic minocycline group, changes in volume values are very low, the amount of size in all areas and lipid contents in intact fat cells was very high (p<0.05). It was followed by systemic drug administiration, local drug administiration and the control group. The maximum number of cells going into apoptosis were observed in control group, the minimum numbers were observed in the systemic and local drug administiration group (p <0.05).
CONCLUSION: In conclusion; degenerative histological changes and “brite adipocyte” formation were seen in 90.days groups due to the stress and the morphological features of white adipose cells were mostly protected by systemic minocyclin administration. Using systemic or local drug administiration is prevent apoptosis in early stages and they increase the number of living fat cells with intact lipid contents. By combining using systemic and local minocycline are potentiated and they effects the retention of fat volume.
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