[doi: 10.5505/2017ichc.PP-171]Investigation Of Protective Effect Of Caffeic Acid Phenethyl Ester On Testicular Damage Which Was Caused By CisplatinTayfun Ceylan1, Emin Kaymak1, Betül Yalçın1, Fazile Cantürk Tan2, Birkan Yakan12Depatment of Biophysics, Erciyes University, Kayseri, Turkey Cisplatin (CP), anticancer drug induces cytotoxicity in healthy tissues. Also cisplatin induces reactive oxygen species and induces apoptosis, inhibiting antioxidant enzymes. Gonadotoxicity is one of the side effects (1-4). Caffeic acid phenethyl ester (CAPE) is Is a component of propolis, a honey bee product (5). Cape used in traditional medicine. CAPE has protective properties for many tissues, has antiviral, anti-inflammatory, immunomodulatory, and antioxidant proporties. CAPE inhibits lipid peroxidation, lipoxygenase and cyclooxygenase enzymes (6-10). The aim of this study has been to evaluate the effects of caffeic acid phenethyl ester on testicular damage induced by cisplatin. Experiment consisted of 40 adult Wistar albino rats, each weighing 200–250 g. Group I served as control and received no drug or agent. Group II received CP intraperitoneally at 7mg/kg for 7 th day. Group III received CAPE 10 µmol/kg /day for 10 days. Group IV received intraperitoneally at 7mg/kg for 7 th day plus CAPE 10 10 µmol/kg /day for 10 days. Testis tissues were processed histopathologically for evaluation of testis injury (testicular Johnsen scores). Anti-oxidant enzymes such as superoxide-dismutase, catalase and the level of malondialdehyde were studied in the testicular tissues of rats with enzyme-linked immunosorbent assay (ELISA). Serum levels of testosterone were studied using ELISA. In the CP group, tubular biopsy score of Johnsen were decreased compared control group. Furthermore, the CAPE treated group showed an improved histological appearance in the CP group. Activities of SOD and CAT in testicular tissues were increased by CP+CAPE and CAPE groups compared control group, but tissue MDA levels was higher than control group. MDA levels were decreased CAPE and CP+CAPE groups. Testosterone levels in CAPE and CP+CAPE groups increased when compared with control group. Our results suggested that CAPE treatment provided protection against cisplatin toxicity. Following CAPE treatment with cisplatin-induced gonadotoxicity, SOD, CAT and testosterone levels increased, while MDA level and testis injury decreased in testis. |