[doi: 10.5505/2017ichc.PP-08]In Vitro Comparison of Cytotoxicity of Gutta Flow Bioseal, Gutta Flow 2, AH Plus and MTA FillapexSuna Karadeniz Saygılı1, Gökhan Saygılı2, Mehmet Ibrahim Tuğlu12İzmir Katip Çelebi Üniversitesi Diş Hekimliği Fakültesi The aim of the study was to evaluate the cytotoxicity of Gutta Flow Bioseal (Coltene Whaledent, GmBHşCo KG,Langenau, Switzerland), Gutta Flow 2 (Coltene Whaledent), AH plus (Dentsply DeTrey, Konstanz, Germany) and MTA Fillapex (Angelus, Londrina, PR, Brazil) on L929 fibroblasts. Samples of the test materials Gutta Flow Bioseal, Gutta Flow 2, AH plus and MTA Fillapex were fabricated in teflon disks of 5 mm diameter and 3 mm thickness. L929 fibroblasts were exposed to extracts for 3 hours, 1 day, 3 and 7 days at 37°C with 5% CO2. Cell viability was evaluated by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium Bromide (MTT). Apoptosis was determined by Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). The data were statistically analysed using analysed by using Kruskal Wallis test for comparison among groups and Dunn multiple comparison post test. The extracts of mixed GuttaFlow Bioseal were nontoxic at all experimental times (p>0,05), whereas the extracts of mixed MTA Fillapex and AH Plus were toxic to fibroblast cells (p<0,001). At 7 days, the number of viable cells in GuttaFlow 2 were more cytotoxic than that of control group and MTA Fillapex was more cytotoxic than AH Plus. The number of apoptosis in MTA Fillapex and AH Plus were more than others for 3 hours interaction (p<0,001). GuttaFlow sealers are less cyototoxic than MTA Fillapex and AH Plus. At all experimental times, the number of viable cells in GuttaFlow Bioseal were no difference statistically compared that of control group. |