[doi: 10.5505/2017ichc.PP-06]The effects of an herbal mixture prepared from Uritica Diogia, Thymus Vulgaris, Glycyrrhiza Glabra, VitisVinifera, Alpinia Officinarum plants’ oil-based extracts on wound healingIrfan Taşdelen1, Şule Ayla2, Mehmet Yalçın Günal3, Kaan Gideroğlu1, Recep Demirhan4, Özge Biçeroğlu22Department of Histology and Embriyology, İstanbul Medipol University School of Medicine, İstanbul, TURKEY. 3Department of Physiology, İstanbul Medipol University School of Medicine, İstanbul, TURKEY. 4Department of Chest Surgery, Dr.Lütfi Kırdar Kartal Research Hospital, İstanbul, TURKEY. A variety of medications and traditional approaches are used improve the process of wound healing. This agent is an herbal mixture and contains oil-based plants extracts from UriticaDiogia, Thymus Vulgaris, GlycyrrhizaGlabra, VitisVinifera, AlpiniaOfficinarum. To investigate this agent that we prepared and standardised on wound healing, we used 28balb-c type mice in total; equally disturbed to four group.The first group is the control group to which we applied sunflower oil (sunflower oil is known to be not effecting the wound healing (reference molecule)). The second group is the comparison group to which we applied Vaseline and the third group is the herbal medication group that Vaseline and the herbal mixture was applied to. Lastly, the fourth group was called Madecassol group to which we applied a medical cream called Madecassol. After wounding the mice, we applied the molecules andthe wound tissues were photographed. From the taken photographs, wound healing rates were calculated in digital measurement programs. After that, the wounded areas were cut out for fixation, and the tissues were buried in the paraffin.Hematoxylin-eosin (HE) and immunohistochemical (VEGF, FGF, collagen type I) staining and scoring were performed on five μm thickness sections from the tissues. In the histopathological scoring, whereas less epidermal and dermal regeneration, medium dense granular tissue, four to five veins in each section were observed in the control and comparison group, in Madecassol and Medicine Group, we saw complete epidermal and dermal regeneration, dense granular tissue and more than seven veins in each section. In the immunohistochemical scoring, a few staining was observed in some of FGF, VGEF and collagen 1 preparations of the control and comparison groups. In those groups were also some preparations which showed no staining. In the herbal medication group, we saw statistically significant increase in staining as well as we observed the same in Madecassol Group. Consequently, our results indicate that the agent that we used can speed the healing process up andwe believe that this agent may be used as a medication after further investigations. |